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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which leads to chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant function in advertising cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already explained. Two further splice variants had been claimed but thorough nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind exclusively towards the goal antibody and block antibody binding. These peptide normally contains the epitope regarded via the antibody.
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This information outlines additional attainable optimization of mobile and immunoassay parameters to ensure the very best success are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies get more info sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin loved ones, whose customers are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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